14 December 2018
A rapid molecular method for detection of CITES-listed shark fins using loop-mediated isothermal amplification (LAMP) has been developed. This rapid detection method would be useful for shark fin traders as well as regulatory agencies to ensure the compliance with and enforcement of CITES-related regulations. A free hands-on training workshop, The Rapid Detection of CITES-listed Sharkfin DNA by Loop-mediated Isothermal Amplification (LAMP) Workshop, was held on 14 December, 2018 to share the developed method to interested parties. Participants from Agriculture, Fisheries and Conservation Department, Government Chinese Medicines Testing Institute, Kadoorie Farm & Botanic Garden, Ocean Park Hong Kong, Ocean Park Conservation Foundation Hong Kong, and academic institutes have joined the workshop. The workshop consisted of a short lecture on the principles and the protocols of using LAMP technology for endangered shark fin identification, and a hands-on laboratory session. Each participant was given the opportunity to carry out a quick and efficient DNA extraction from shark fin sample, followed by setting up their LAMP reactions. There was also a lively discussion among all the participants, who shared their experience in onsite molecular identification and conservation. The workshop has received an unanimously positive feedback from the participants. All participants agreed or strongly agreed that he/she had learnt the skills of DNA extraction and setting up LAMP reactions for identification of endangered shark fins.
For the photos of the workshop, please click the following link: